Protocol

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Protocol

Supporting Information of Molecular biology 1

Plasmids

DNA Sequences

Primers

 Hint:

1-14 are used to Construct plasmids by Gibson Assembly

15-16 are used to add Restriction Site by PCR (HindIII and NheI)

All primers are designed with the help of SnapGene.

Reference

[1] Li S, Sun T, Xu C, Chen L, Zhang W. 2018. Development and optimization of genetic toolboxes for a fast-growing cyanobacterium Synechococcus elongatus UTEX 2973. Metabolic Engineering. 48:163-174. doi:10.1016/j.ymben.2018.06.002.
[2] Li T, Li C-T, Butler K, Hays S, Guarnieri M, Oyler G, Betenbaugh M. 2017. Mimicking lichens: Incorporation of yeast strains together with sucrose-secreting cyanobacteria improves survival, growth, ROS removal, and lipid production in a stable mutualistic co-culture production platform. Biotechnology for Biofuels. 10. doi:10.1186/s13068-017-0736-x.
[3] Lin P-C, Zhang F, Pakrasi HB. 2020. Enhanced production of sucrose in the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973. Scientific Reports. 10(1):390. doi:10.1038/s41598-019-57319-5.
[4] Hays SG, Yan LLW, Silver PA, Ducat DC. Synthetic photosynthetic consortia define interactions leading to robustness and photoproduction. J Biol Eng. 2017 Jan 23;11:4. doi: 10.1186/s13036-017-0048-5. PMID: 28127397; PMCID: PMC5259876.